- MA5-12680 - Invitrogen Antibodies IL2RA antibody

Submitted references Modulation of PTPN2/22 Function by Spermidine in CRISPR-Cas9-Edited T-Cells Associated with Crohn's Disease and Rheumatoid Arthritis.

Shaw AM, Qasem A, Naser SA
International journal of molecular sciences 2021 Aug 18;22(16)

A kinetic investigation of interacting, stimulated T cells identifies conditions for rapid functional enhancement, minimal phenotype differentiation, and improved adoptive cell transfer tumor eradication.

Zhou J, Bethune MT, Malkova N, Sutherland AM, Comin-Anduix B, Su Y, Baltimore D, Ribas A, Heath JR
PloS one 2018;13(1):e0191634

Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer.

Costa A, Kieffer Y, Scholer-Dahirel A, Pelon F, Bourachot B, Cardon M, Sirven P, Magagna I, Fuhrmann L, Bernard C, Bonneau C, Kondratova M, Kuperstein I, Zinovyev A, Givel AM, Parrini MC, Soumelis V, Vincent-Salomon A, Mechta-Grigoriou F
Cancer cell 2018 Mar 12;33(3):463-479.e10

CD25 is a novel marker of hepatic bile canaliculus.

Lu ZH, -Chen W, Ju CX, -Den J, Kuai SG, Pei H, Huang LH, Gu XB, Ying YX, Hu XQ, Zhu TF, Xing YP, Fan QH
International journal of surgical pathology 2012 Oct;20(5):455-61

FOXP3 T regulatory cells in lesions of oral lichen planus correlated with disease activity.

Tao XA, Xia J, Chen XB, Wang H, Dai YH, Rhodus NL, Cheng B
Oral diseases 2010 Jan;16(1):76-82

Regulatory T cells and liver pathology in a murine graft versus host response model.

Miyazaki T, Doy M, Unno R, Honda A, Ikegami T, Itoh S, Bouscarel B, Matsuzaki Y
Hepatology research : the official journal of the Japan Society of Hepatology 2009 Jun;39(6):585-94

Adherens junctions in myelinating Schwann cells stabilize Schmidt-Lanterman incisures via recruitment of p120 catenin to E-cadherin.

Tricaud N, Perrin-Tricaud C, Brusés JL, Rutishauser U
The Journal of neuroscience : the official journal of the Society for Neuroscience 2005 Mar 30;25(13):3259-69

Genetic engineering of glomerular sclerosis in the mouse via control of onset and severity of podocyte-specific injury.

Matsusaka T, Xin J, Niwa S, Kobayashi K, Akatsuka A, Hashizume H, Wang QC, Pastan I, Fogo AB, Ichikawa I
Journal of the American Society of Nephrology : JASN 2005 Apr;16(4):1013-23

Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src.

Willey CD, Balasubramanian S, Rodríguez Rosas MC, Ross RS, Kuppuswamy D
Journal of molecular and cellular cardiology 2003 Jun;35(6):671-83

Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src.

Willey CD, Balasubramanian S, Rodríguez Rosas MC, Ross RS, Kuppuswamy D
Journal of molecular and cellular cardiology 2003 Jun;35(6):671-83

Association of increased levels of heavy-chain ferritin with increased CD4+ CD25+ regulatory T-cell levels in patients with melanoma.

Gray CP, Arosio P, Hersey P
Clinical cancer research : an official journal of the American Association for Cancer Research 2003 Jul;9(7):2551-9

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD25 (green) showing staining in the cytoplasm of BAF-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD25 monoclonal antibody (Product # MA5-12680) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of CD25 showing positive staining in the membrane of paraffin-treated Human spleen tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CD25 monoclonal antibody (Product # MA5-12680) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of CD25 showing positive staining in the membrane of paraffin-treated Human tonsil tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CD25 monoclonal antibody (Product # MA5-12680) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD25 in BAF-3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD25 monoclonal antibody (Product # MA5-12680) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD25 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD25 monoclonal antibody (Product # MA5-12680) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

MA5-12680

Antibody data