MAB3228

antibody from Abnova Corporation

Targeting: IL2RA CD25, IDDM10, IL2R

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3228

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB3228, RRID:AB_10552654

Product name

Il2ra monoclonal antibody, clone 7D4

Antibody type

Monoclonal

Description

Rat monoclonal antibody raised against Il2ra.

Isotype

IgM

Antibody clone number

7D4

Storage

Store at 4°C.

References

Submitted references

IL-2 receptor alpha chain expression during early B lymphocyte differentiation.

Chen J, Ma A, Young F, Alt FW
International immunology 1994 Aug;6(8):1265-8

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IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development.

Rolink A, Grawunder U, Winkler TH, Karasuyama H, Melchers F
International immunology 1994 Aug;6(8):1257-64

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Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site.

Moreau JL, Nabholz M, Diamantstein T, Malek T, Shevach E, Thèze J
European journal of immunology 1987 Jul;17(7):929-35

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Cells from BALB/c spleen were activated by PMA (10 ng/mL) and ionomycin (400 ng/mL) for 48 hours, then stained with Il2ra monoclonal antibody, clone 7D4 (Cat # MAB3228)-FITC and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).

Validation comment

Flow Cytometry