Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 124232 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#124232, RRID:AB_2616771
- Product name
- Alexa Fluor® 594 anti-mouse/rat/human CD27
- Antibody type
- Monoclonal
- Antigen
- Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
- Reactivity
- Human, Mouse, Rat
- Conjugate
- Yellow dye
- Antibody clone number
- LG.3A10
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- C57BL/6 mouse frozen spleen sections were fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat serum for one hour at room temperature. Then the section was stained with 5 µg/ml of CD27 (clone LG.3A10) Alexa Fluor® 594 (red), 2.5 µg/ml of B220 (clone RA3-6B2) Brilliant Violet 421™ (blue), CD4 (clone GK1.5) Alexa Fluor® 488 (green) and CD8 (clone 53-6.7) Alexa Fluor® 647 (yellow) overnight at 4°C. The image was captured by 10X objective.
- Conjugate
- Yellow dye