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LearnA700-083-T
antibody from Invitrogen Antibodies
Targeting: CD27
S152, TNFRSF7, Tp55
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- A700-083-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD27 Recombinant Rabbit Monoclonal Antibody (BLR083G)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR083G
- Vial size
- 10 µL
- Concentration
- 500 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD27 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, Ramos, HEK293T, Raji, Jurkat, K-562, U2OS, Daudi, MCF-7, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD27 recombinant monoclonal antibody [BLR083G] (Product # A700-083 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH (Product # A300-639A).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD27 in FFPE Ramos cells by ICC. Antibody: Rabbit anti-CD27 recombinant monoclonal antibody [BLR083G] (Product # A700-083 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD27 in FFPE tonsil by IHC. Antibody: Rabbit anti-CD27 recombinant monoclonal antibody (BLR083G) (A700-083 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD27 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-CD27 recombinant monoclonal [BLR083G] (Product #A700-083) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD27 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD27 recombinant monoclonal antibody [BLR083G] (Product # A700-083 lot 1) used for IP at 20 µL/mg lysate. CD27 was also immunoprecipitated by rabbit anti-CD27 antibody BL16148. For blotting immunoprecipitated CD27 (Product # A700-083) was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
