MAB6912

antibody from Abnova Corporation

Targeting: CD40 Bp50, p50, TNFRSF5

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6912

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB6912, RRID:AB_10680301

Product name

CD40 monoclonal antibody, clone EA-5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against native CD40.

Isotype

IgG

Antibody clone number

EA-5

Storage

Store at 4°C.

References

Submitted references

Follicular dendritic cells inhibit apoptosis in human B lymphocytes by a rapid and irreversible blockade of preexisting endonuclease.

Lindhout E, Lakeman A, de Groot C
The Journal of experimental medicine 1995 Jun 1;181(6):1985-95

---

Cross-linking CD40 on human B cell precursors inhibits or enhances growth depending on the stage of development and the IL costimulus.

Larson AW, LeBien TW
Journal of immunology (Baltimore, Md. : 1950) 1994 Jul 15;153(2):584-94

---

Soluble forms of CD40 inhibit biologic responses of human B cells.

Fanslow WC, Anderson DM, Grabstein KH, Clark EA, Cosman D, Armitage RJ
Journal of immunology (Baltimore, Md. : 1950) 1992 Jul 15;149(2):655-60

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

5 X 10⁵ cultured Raji human tumor cells were incubated 45 minutes on ice with 80 uL of CD40 monoclonal antibody, clone EA-5 (Cat # MAB6912) at 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.64 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.

Validation comment

Flow Cytometry