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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS4679 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- E2F1 (phospho T433) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human E2F1 around the phosphorylation site of Threonine 433.
- Description
- The E2F transcription factors are essential for regulation of the cell cycle. Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit. Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control. E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-E2F1 (T433) pAb at 1:500 dilutionLane1:Jurkat cell lysate treated with colchicine(0.2¦Ìg/ML,24h)Lane2:Mouse spleen tissue lysateLane3:PC12 cell lysate treated with colchicine(0.2¦Ìg/ML,24h)
