LS-C677745

antibody from LSBio

Targeting: E2F1 RBBP3, RBP3

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C677745

Provider

LSBio

Product name

E2F1 Antibody LS-C677745

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating E2F1 in 3T3 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of product in 3T3 whole cell lysate.For western blotting,a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product (8µg) + 3T3 whole cell lysate (500µg) Lane 3: 3T3 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating E2F1 in 3T3 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of product in 3T3 whole cell lysate.For western blotting,a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product (8µg) + 3T3 whole cell lysate (500µg) Lane 3: 3T3 whole cell lysate (10µg)