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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- MA5-14341 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-E2F1 Monoclonal Antibody (KH95), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-14344 targets E2F-1 Transcription Factor in gel shift, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Human and mouse samples. The MA5-14344 immunogen is recombinant human E2F-1 protein.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- KH95
- Vial size
- 500 µL
- Concentration
- 0.2 mg/ml
- Storage
- 4° C
Submitted references The relationship between E2F family members and tumor growth in colorectal adenocarcinomas: A comparative immunohistochemical study of 100 cases.
CDK4/6 inhibition antagonizes the cytotoxic response to anthracycline therapy.
Enhanced expression of 70-kilodalton heat shock protein limits cell division in a sepsis-induced model of acute respiratory distress syndrome.
Deregulated expression of p16INK4a and p53 pathway members in benign and malignant myoepithelial tumours of the salivary glands.
Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers.
Expression of E2F-4 gene in colorectal adenocarcinoma and corresponding covering mucosa: an immunohistochemistry, image analysis, and immunoblot study.
UCN-01 suppresses E2F-1 mediated by ubiquitin-proteasome-dependent degradation.
Xanthoulis A, Kotsinas A, Tiniakos D, Fiska A, Tentes AA, Kyroudi A, Kittas C, Gorgoulis V
Applied immunohistochemistry & molecular morphology : AIMM 2014 Jul;22(6):471-7
Applied immunohistochemistry & molecular morphology : AIMM 2014 Jul;22(6):471-7
CDK4/6 inhibition antagonizes the cytotoxic response to anthracycline therapy.
McClendon AK, Dean JL, Rivadeneira DB, Yu JE, Reed CA, Gao E, Farber JL, Force T, Koch WJ, Knudsen ES
Cell cycle (Georgetown, Tex.) 2012 Jul 15;11(14):2747-55
Cell cycle (Georgetown, Tex.) 2012 Jul 15;11(14):2747-55
Enhanced expression of 70-kilodalton heat shock protein limits cell division in a sepsis-induced model of acute respiratory distress syndrome.
Bromberg Z, Raj N, Goloubinoff P, Deutschman CS, Weiss YG
Critical care medicine 2008 Jan;36(1):246-55
Critical care medicine 2008 Jan;36(1):246-55
Deregulated expression of p16INK4a and p53 pathway members in benign and malignant myoepithelial tumours of the salivary glands.
Vékony H, Röser K, Löning T, Raaphorst FM, Leemans CR, Van der Waal I, Bloemena E
Histopathology 2008 Dec;53(6):658-66
Histopathology 2008 Dec;53(6):658-66
Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers.
Au NH, Cheang M, Huntsman DG, Yorida E, Coldman A, Elliott WM, Bebb G, Flint J, English J, Gilks CB, Grimes HL
The Journal of pathology 2004 Sep;204(1):101-9
The Journal of pathology 2004 Sep;204(1):101-9
Expression of E2F-4 gene in colorectal adenocarcinoma and corresponding covering mucosa: an immunohistochemistry, image analysis, and immunoblot study.
Mady HH, Hasso S, Melhem MF
Applied immunohistochemistry & molecular morphology : AIMM 2002 Sep;10(3):225-30
Applied immunohistochemistry & molecular morphology : AIMM 2002 Sep;10(3):225-30
UCN-01 suppresses E2F-1 mediated by ubiquitin-proteasome-dependent degradation.
Hsueh CT, Wu YC, Schwartz GK
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Mar;7(3):669-74
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Mar;7(3):669-74
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with E2F-1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of the cells.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of E2F-1 Transcription Factor using E2F-1 Transcription Factor Monoclonal Antibody (Product # MA5-14341) on Native Mouse MAD109 Cells.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of E2F-1 Transcription Factor using E2F-1 Transcription Factor Monoclonal Antibody (Product # MA5-14341) on denatured Mouse MAD109 Cells.
- Conjugate
- Biotin
