PB9512

antibody from Boster Biological Technology

Targeting: STAT5B

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PB9512

Provider

Boster Biological Technology

Product name

Anti-STAT5b Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for STAT5B detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. STAT5B information: Molecular Weight: 89866 MW; Subcellular Localization: Cytoplasm . Nucleus . Translocated into the nucleus in response to phosphorylation.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of Jurkat cells using anti-STAT5b antibody (PB9512). Overlay histogram showing Jurkat cells stained with PB9512 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT5b Antibody (PB9512, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image