LS-C186112

antibody from LSBio

Targeting: XRCC5 KARP-1, KU80, Ku86, KUB2

Western blot

Antibody data

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Validation data

Product number: LS-C186112 - Provider product page
Provider: LSBio
Product name: XRCC5 / Ku80 Antibody (aa315-593) LS-C186112
Antibody type: Polyclonal
Description: Immunoaffinity purified
Reactivity: Human
Host: Rabbit
Storage: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

XRCC5 protein structure - LS-C186112 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Ku80 (XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 ug of Ku80 antibody 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Ku80 (XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 ug of Ku80 antibody 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Ku80 (XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 ug of Ku80 antibody 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Ku80 (XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 ug of Ku80 antibody 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.