16389-1-AP

antibody from Proteintech Group

Targeting: XRCC5 KARP-1, KU80, Ku86, KUB2

Provider product page for 16389-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [16]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 16389-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16389-1-AP, RRID:AB_2257509
Product name: XRCC5/Ku80 antibody
Antibody type: Polyclonal
Description: KD/KO validated XRCC5/Ku80 antibody (Cat. #16389-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

XRCC5 protein structure - 16389-1-AP shown in red.

Submitted references Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway.

Wang L, Huang Y, Liu C, Guo M, Ma Z, He J, Wang A, Sun X, Liu Z
Journal of Cancer 2021;12(3):860-873

Epithelial cell transforming factor ECT2 is an important regulator of DNA double-strand break repair and genome stability.

Cao C, Han P, Liu L, Tang Y, Tian S, Zhang K, Shi L, Liu Z, Zhuo D, Ge W, Gong W
The Journal of biological chemistry 2021 Sep;297(3):101036

Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca²⁺]_m-PDP1-PDH-histone acetylation retrograde signaling.

Shi Y, Wang Y, Jiang H, Sun X, Xu H, Wei X, Wei Y, Xiao G, Song Z, Zhou F
Cell death & disease 2021 Sep 6;12(9):837

Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shortening.

Tichy ED, Ma N, Sidibe D, Loro E, Kocan J, Chen DZ, Khurana TS, Hasty P, Mourkioti F
Cell reports 2021 May 11;35(6):109098

Elevated XRCC5 expression level can promote temozolomide resistance and predict poor prognosis in glioblastoma.

Lee IN, Yang JT, Huang C, Huang HC, Wu YP, Chen JC
Oncology letters 2021 Jun;21(6):443

OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair.

Cui Y, Xie R, Zhang X, Liu Y, Hu Y, Li Y, Liu X, Yu X, Wu C
Cell death & disease 2021 Jun 16;12(7):622

LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma.

Guo Z, Wang YH, Xu H, Yuan CS, Zhou HH, Huang WH, Wang H, Zhang W
Cell death & disease 2021 Jan 4;12(1):69

Cytoplasmic DNA sensing by KU complex in aged CD4⁺ T cell potentiates T cell activation and aging-related autoimmune inflammation.

Wang Y, Fu Z, Li X, Liang Y, Pei S, Hao S, Zhu Q, Yu T, Pei Y, Yuan J, Ye J, Fu J, Xu J, Hong J, Yang R, Hou H, Huang X, Peng C, Zheng M, Xiao Y
Immunity 2021 Apr 13;54(4):632-647.e9

DNA methylation‑regulated miR‑155‑5p depresses sensitivity of esophageal carcinoma cells to radiation and multiple chemotherapeutic drugs via suppression of MAP3K10.

Luo W, Zhang H, Liang X, Xia R, Deng H, Yi Q, Lv L, Qian L
Oncology reports 2020 May;43(5):1692-1704

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

Li G, Zhang X, Wang H, Liu D, Li Z, Wu Z, Yang H
The international journal of biochemistry & cell biology 2020 Aug;125:105790

Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes.

Martin JH, Bromfield EG, Aitken RJ, Lord T, Nixon B
Scientific reports 2018 Jun 26;8(1):9685

Flavone protects HBE cells from DNA double-strand breaks caused by PM2.5.

Ren X, Tang Y, Sun J, Feng J, Chen L, Chen H, Zeng S, Chen C, Li X, Zhu H, Zeng Z
Human cell 2018 Apr;31(2):116-126

Downregulation of Mitochondrial Single Stranded DNA Binding Protein (SSBP1) Induces Mitochondrial Dysfunction and Increases the Radiosensitivity in Non-Small Cell Lung Cancer Cells.

Wang Y, Hu L, Zhang X, Zhao H, Xu H, Wei Y, Jiang H, Xie C, Zhou Y, Zhou F
Journal of Cancer 2017;8(8):1400-1409

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Hu L, Li X, Liu Q, Xu J, Ge H, Wang Z, Wang H, Wang Z, Shi C, Xu X, Huang J, Lin Z, Pieper RO, Weng C
Oncogene 2017 Feb 23;36(8):1145-1156

Sensitization of tamoxifen-resistant breast cancer cells by Z-ligustilide through inhibiting autophagy and accumulating DNA damages.

Qi H, Jiang Z, Wang C, Yang Y, Li L, He H, Yu Z
Oncotarget 2017 Apr 25;8(17):29300-29317

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Dong H, Shi Q, Song X, Fu J, Hu L, Xu D, Su C, Xia X, Song E, Song Y
Toxicology and applied pharmacology 2015 Jul 1;286(1):10-6

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 16389-1-AP(XRCC5 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using XRCC5 antibody 16389-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 16389-1-AP(XRCC5 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 16389-1-AP(XRCC5 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue