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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX109935 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109935, RRID:AB_1952614
- Product name
- Ku80 antibody [N3C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references PHRF1 promotes genome integrity by modulating non-homologous end-joining.
Non-homologous end joining pathway is the major route of protection against 4β-hydroxywithanolide E-induced DNA damage in MCF-7 cells.
Effect of DNA damage response by quinazolinone analogue HMJ-38 on human umbilical vein endothelial cells: evidence for γH2A.X and DNA-PK-dependent pathway.
Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin.
Chang CF, Chu PC, Wu PY, Yu MY, Lee JY, Tsai MD, Chang MS
Cell death & disease 2015 Apr 9;6:e1716
Cell death & disease 2015 Apr 9;6:e1716
Non-homologous end joining pathway is the major route of protection against 4β-hydroxywithanolide E-induced DNA damage in MCF-7 cells.
You BJ, Wu YC, Lee CL, Lee HZ
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2014 Mar;65:205-12
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2014 Mar;65:205-12
Effect of DNA damage response by quinazolinone analogue HMJ-38 on human umbilical vein endothelial cells: evidence for γH2A.X and DNA-PK-dependent pathway.
Chiang JH, Yang JS, Lu CC, Hour MJ, Liu KC, Lin JH, Lee TH, Chung JG
Human & experimental toxicology 2014 Jun;33(6):590-601
Human & experimental toxicology 2014 Jun;33(6):590-601
Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin.
Masuda Y, Noguchi K, Segawa H, Tanaka N, Katayama K, Mitsuhashi J, Sugimoto Y
Biochemical and biophysical research communications 2011 Nov 18;415(2):305-12
Biochemical and biophysical research communications 2011 Nov 18;415(2):305-12
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ku80 antibody [N3C2], Internal detects XRCC5 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract 7.5% SDS-PAGEKu80 antibody [N3C2], Internal (GTX109935) dilution: 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ku80 antibody [N3C2], Internal detects Ku80 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ku80 protein stained by Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ku80(XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 £gg of Ku80 antibody (GTX109935) 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody (GTX109935) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Ku80 (XRCC5)(GTX109935) antibody at 1:100 dilution.
