H00005048-M03

antibody from Abnova Corporation

Targeting: PAFAH1B1 LIS1, MDCR, MDS, NudF, PAFAH

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005048-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005048-M03, RRID:AB_1578367

Product name

PAFAH1B1 monoclonal antibody (M03), clone 5A5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PAFAH1B1.

Antigen sequence

MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEA
ELDVNEELDKKYAGLLEKKWTSVIRLQKKVMELES
KLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALS
GHRSP


Isotype

IgG

Antibody clone number

5A5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5.Lane 1: PAFAH1B1 transfected lysate(46.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PAFAH1B1 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PAFAH1B1 on HeLa cell . [antibody concentration 15 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol