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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX102640 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102640, RRID:AB_11169314
- Product name
- Topoisomerase II beta antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2.
Systematic polypharmacology and drug repurposing via an integrated L1000-based Connectivity Map database mining.
Hangas A, Aasumets K, Kekäläinen NJ, Paloheinä M, Pohjoismäki JL, Gerhold JM, Goffart S
Nucleic acids research 2018 Oct 12;46(18):9625-9636
Nucleic acids research 2018 Oct 12;46(18):9625-9636
Systematic polypharmacology and drug repurposing via an integrated L1000-based Connectivity Map database mining.
Liu TP, Hsieh YY, Chou CJ, Yang PM
Royal Society open science 2018 Nov;5(11):181321
Royal Society open science 2018 Nov;5(11):181321
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Jurkat 5% SDS PAGE GTX102640 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Topoisomerase II beta antibody immunoprecipitates Topoisomerase II beta protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 £gg of Topoisomerase II beta antibody (GTX102640) 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody (GTX102640) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Topoisomerase II beta(GTX102640) antibody at 1:250 dilution.
