CABT-B9344

antibody from Creative Diagnostics

Targeting: TOP2B

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

CABT-B9344

Provider

Creative Diagnostics

Product name

Mouse anti-Human Topo IIÉÇ monoclonal antibody, clone 50/Upqp JJÉÇ

Antibody type

Monoclonal

Description

Eukaryotic DNA topoisomerase II, a ubiquitous ATP-dependent type II topoisomerase, is an essential nuclear enzyme in DNA replication and transcription, chromatin segregation, and cell cycle progression. Topoisomerases transiently break a pair of complementary strands in double-stranded DNA to form a gate for the passage of duplex DNA. Two isoforms of DNA topoisomerase II have been identified: topo IIÏ« and topo IIÉÇ. These exhibit a high degree of homology, except for some divergence in the C-terminal region. Both contain multiple bipartite nuclear localization sequences (NLS) that mediate their subnuclear localization. Topo IIÏ« levels rise during late S phase and peak in G2-M, whereas topo IIÉÇ levels remain constant throughout the cell cycle. In addition, topo IIÏ« is expressed in proliferating cells, while topo IIÉÇ is expressed in a wide range of tissues. Although the exact role of these two isoforms during cell proliferation is not known, the differences in cellular expression implicate different physiological roles. Both isoforms may also be important targets for anticancer agents that exert cytotoxicity in proliferating cells via stabilization of a topo II-DNA complex.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

50/Upqp JJÉÇ

Vial size

50ug, 150ug

Concentration

250 Ã×g/ml

Storage

Store undiluted at -20°C.