PA5-27750

antibody from Invitrogen Antibodies

Targeting: TOP2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27750

Provider

Invitrogen Antibodies

Product name

TOP2B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, K562, THP-1. Predicted reactivity: Mouse (89%), Rat (86%), Pig (88%), Rhesus Monkey (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.48 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TOP2B was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a TOP2B Polyclonal Antibody (Product # PA5-27750) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TOP2B was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a TOP2B Polyclonal Antibody (Product # PA5-27750) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TOP2B Polyclonal Antibody (Product # PA5-27750). Sample (30 µg of whole cell lysate). Lane A: Jurkat. 5% SDS PAGE. TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TOP2B was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Topoisomerase II beta (Product # PA5-27750) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TOP2B Polyclonal Antibody detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Topoisomerase II beta stained by TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TOP2B Polyclonal Antibody detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Topoisomerase II beta stained by TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TOP2B Polyclonal Antibody detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Topoisomerase II beta stained by TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Topoisomerase II beta antibody immunoprecipitates Topoisomerase II beta protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 µg of Topoisomerase II beta antibody (Product # PA5-27750) 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody (Product # PA5-27750) diluted at 1:1,000.