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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA1-16928 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRE1 alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody does not detect monkey IRE1 alpha.
- Concentration
- 1 mg/mL
Submitted references Attenuation of unfolded protein response and apoptosis by mReg2 induced GRP78 in mouse insulinoma cells.
Liu L, Chowdhury S, Fang X, Liu JL, Srikant CB
FEBS letters 2014 May 29;588(11):2016-24
FEBS letters 2014 May 29;588(11):2016-24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of Ire1 alpha in transfected Min6 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRE1 alpha in Min6 cells transfected with GFP-siRNA or Ire1-siRNA. Samples were incubated in IRE1 alpha polyclonal antibody (Product # PA1-16928). Theoretical molecular weight: 110 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IRE1 alpha in formalin fixed mouse colon tissue section. Samples were incubated in IRE1 alpha polyclonal antibody (Product # PA1-16928) using a dilution of 1:300. Note a strong cytoplasmic signal in various cells of colonic villi (columnar epithelial and goblet cells) and the muscular layer. A few cells, potentially with active UPR, showed nuclear positivity also while the signal was negligible in cells at base of crypts and lamina propria.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IRE1 alpha in formalin fixed mouse colon tissue section. Samples were incubated in IRE1 alpha polyclonal antibody (Product # PA1-16928) using a dilution of 1:300. Note a strong cytoplasmic signal in various cells of colonic villi (columnar epithelial and goblet cells) and the muscular layer. Some cells, most likely with active UPR, depicted nuclear positivity also while the signal was negligible in crypts cells and lamina propria.
