NBP1-43299-0.1mg

antibody from Novus Biologicals

Targeting: TBX21 T-bet, TBLYM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NBP1-43299-0.1mg

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-43299-0.1mg, RRID:AB_10006648

Product name

Mouse Monoclonal T-bet/TBX21 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: T-bet/TBX21 Antibody (39D) [NBP1-43299] - CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP.