PA5-27281

antibody from Invitrogen Antibodies

Targeting: RPSA 37LRP, LAMR1, LRP, p40, SA

Antibody data

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Validation data

Product number: PA5-27281 - Provider product page
Provider: Invitrogen Antibodies
Product name: RPSA Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HepG2, Jurkat, Mouse brain. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (93%), Xenopus laevis (95%), Dog (100%), Pig (100%), Chicken (98%), Sheep (99%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RPSA protein structure - PA5-27281 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RPSA using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RPSA polyclonal antibody (Product # PA5-27281) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RPSA was performed by separating 30 µg of Jurkat lysates by 12% SDS PAGE. Proteins were transferred to a membrane and probed with a RPSA Polyclonal Antibody (Product # PA5-27281) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RPSA Polyclonal Antibody (Product # PA5-27281). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. RPSA Polyclonal Antibody (Product # PA5-27281) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RPSA Polyclonal Antibody (Product # PA5-27281). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA Polyclonal Antibody (Product # PA5-27281) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RPSA was performed by separating 30 µg of whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RPSA Polyclonal Antibody (Product # PA5-27281) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: RPSA antibody detects RPSA protein by Western blot analysis. Mouse tissue extracts (50 µg) was separated by 10 % SDS-PAGE, and the membrane was blotted with RPSA antibody RPSA Polyclonal Antibody (Product # PA5-27281) diluted by 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: RPSA antibody detects RPSA protein by Western blot analysis. Whole cell extracts (30 µg) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody RPSA Polyclonal Antibody (Product # PA5-27281) diluted by 1:1,000.