16200-1-AP

antibody from Invitrogen Antibodies

Targeting: ALAS1 ALAS, ALAS3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16200-1-AP

Provider

Invitrogen Antibodies

Product name

ALAS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MESVVRRCP FLSRVPQAFL QKAGKSLLFY AQNCPKMMEV GAKPAPRALS TAAVHYQQIK ETPPASEKDK TAKAKVQQTP DGSQQSPDGT QLPSGHPLPA TSQGTASKCP FLAAQMNQRG SSVFCKASLE LQEDVQEMNA VRKEVAETSA GPSVVSVKTD GGDPSGLLKN FQDIMQKQRP ERVSHLLQDN LPKSVSTFQY DRFFEKKIDE KKNDHTYRVF KTVNRRAHIF PMADDYSDSL ITKKQVSVWC SNDYLGMSRH PRVCGAVMDT LKQHGAGAGG TRNISGTSKF HVDLERELAD LHGKDAALLF SSCFVANDST LFTLAKMMPG C (1-330 aa encoded by BC011798)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16200-1-AP ( ALAS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 16200-1-AP (ALAS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ALAS1 (IP:16200-1-AP, 4ug; Detection:16200-1-AP 1:300) with HepG2 cells lysate 3120ug.