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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-56415 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56415, RRID:AB_2225623
- Product name
- Rabbit Polyclonal Alas1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Nuclear receptor 5A (NR5A) family regulates 5-aminolevulinic acid synthase 1 (ALAS1) gene expression in steroidogenic cells.
Gonadotropin regulation of genes differentially expressed in response to PKCzeta inhibitor during ovulation in the rat.
Differential regulation of human ALAS1 mRNA and protein levels by heme and cobalt protoporphyrin.
Ju Y, Mizutani T, Imamichi Y, Yazawa T, Matsumura T, Kawabe S, Kanno M, Umezawa A, Kangawa K, Miyamoto K
Endocrinology 2012 Nov;153(11):5522-34
Endocrinology 2012 Nov;153(11):5522-34
Gonadotropin regulation of genes differentially expressed in response to PKCzeta inhibitor during ovulation in the rat.
Seo YM, Park JI, Jeon MJ, Park EW, Park JK, Lee HC, Chang WK, Chun SY
Life sciences 2009 Jul 17;85(3-4):153-60
Life sciences 2009 Jul 17;85(3-4):153-60
Differential regulation of human ALAS1 mRNA and protein levels by heme and cobalt protoporphyrin.
Zheng J, Shan Y, Lambrecht RW, Donohue SE, Bonkovsky HL
Molecular and cellular biochemistry 2008 Dec;319(1-2):153-61
Molecular and cellular biochemistry 2008 Dec;319(1-2):153-61
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Alas1 Antibody [NB100-56415] - analysis of HepG2 cell lysate using ALAS1 antibody at1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Alas1 Antibody [NB100-56415] - Simple Western lane view shows a specific band for Alas1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Alas1 Antibody [NB100-56415] - Staining at 5 ug/ml. Heat induced antigen retrieval in pH 6.0 citrate buffer was used for unmasking. A biotinylated secondary antibody followed by alkaline phosphatase-streptavidin was and chromogen was used for detection.
