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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunoprecipitation [1]
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- Product number
- PAB10091 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10091, RRID:AB_1672731
- Product name
- Angpt2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Angpt2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis.
Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis.
Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning.
Maisonpierre PC, Suri C, Jones PF, Bartunkova S, Wiegand SJ, Radziejewski C, Compton D, McClain J, Aldrich TH, Papadopoulos N, Daly TJ, Davis S, Sato TN, Yancopoulos GD
Science (New York, N.Y.) 1997 Jul 4;277(5322):55-60
Science (New York, N.Y.) 1997 Jul 4;277(5322):55-60
Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis.
Suri C, Jones PF, Patan S, Bartunkova S, Maisonpierre PC, Davis S, Sato TN, Yancopoulos GD
Cell 1996 Dec 27;87(7):1171-80
Cell 1996 Dec 27;87(7):1171-80
Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning.
Davis S, Aldrich TH, Jones PF, Acheson A, Compton DL, Jain V, Ryan TE, Bruno J, Radziejewski C, Maisonpierre PC, Yancopoulos GD
Cell 1996 Dec 27;87(7):1161-9
Cell 1996 Dec 27;87(7):1161-9
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Immunoblotting of Angpt2 polyclonal antibody (Cat # PAB10091) was used at a 1:500 dilution to detect mouse Angpt2 in supernatants of mouse-angiopoietin-expressing endothelial cells.Lane 1 - mock endothelial cells.Lane 2 - mouse Angptl2 (clone 2-9) expressing cells.Lane 3 - mouse Angpt1 (clone 1-15) expressing cells.Lane 4 - mouse Angpt1 (clone 1-8) expressing cells.Lane 5 - wt endothelial cells.Approximately 20 ug of each lysate was loaded for 10% SDS-PAGE.Immunoprecipitation preceded reaction with primary antibody at room temperature for 1 h.After subsquent washing, a 1 : 5000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.
- Validation comment
- Immunoprecipitation
