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antibody from Invitrogen Antibodies
Targeting: TCF3
bHLHb21, E2A, E47, ITF1, MGC129647, MGC129648, p75, VDIR
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
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- Product number
- PA5-20900 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TCF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human brain tissue lysate.
- Concentration
- 1 mg/mL
Submitted references The Transcription Factor SCX is a Potential Serum Biomarker of Fibrotic Diseases.
Ramírez-Aragón M, Hernández-Sánchez F, Rodríguez-Reyna TS, Buendía-Roldán I, Güitrón-Castillo G, Núñez-Alvarez CA, Hernández-Ramírez DF, Benavides-Suárez SA, Esquinca-González A, Torres-Machorro AL, Mendoza-Milla C
International journal of molecular sciences 2020 Jul 16;21(14)
International journal of molecular sciences 2020 Jul 16;21(14)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human brain tissue lysate using a TCF3 polyclonal antibody (Product # PA5-20900) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TCF3 in Human brain tissue lysate with TCF3 Polyclonal Antibody (Product # PA5-20900) at 1 µg/mL in (A) the absence and (B) presence of peptide blocking.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of TCF3 was achieved by transfecting Daudi with TCF3 specific siRNAs (Silencer® select Product # s13876, s224712). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) (20 µg lysate) from the TCF3 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with TCF3 Polyclonal Antibody (Product # PA5-20900, 1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TCF3
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TCF3 Polyclonal Antibody (Product # PA5-20900) and a 68kDa band corresponding to TCF3 was observed across all cell lines tested. Modified whole cell extracts (1% SDS) (50 µg lysate) of Ramos (Lane 1), Jurkat (Lane 2), KARPAS 299 (Lane 3), K-562 (Lane 4) and Daudi (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TCF3 in rat brain tissue with TCF3 Polyclonal Antibody (Product # PA5-20900) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TCF3 in rat brain tissue with TCF3 Polyclonal Antibody (Product # PA5-20900) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TCF3 in rat liver tissue with TCF3 Polyclonal Antibody (Product # PA5-20900) at 5 µg/mL.
