Explore
Validate
LearnMAB6360
antibody from R&D Systems
Targeting: LRP1
A2MR, APOER, APR, CD91, IGFBP-3R, IGFBP3R1, LRP, LRP1A
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB6360 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LRP-1 Intracellular Domain Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human LRP-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) LRP-1 clusters 2, 3, or 4, rhLRP-4, rhLRP-5, rhLRP-5 intracellular domain, rhLRP-6, rhLRP-6 intracellular domain, or rhLRPAP is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q07954- Isotype
- IgG
- Antibody clone number
- 545503
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A potential neuroprotective role of apolipoprotein E-containing lipoproteins through low density lipoprotein receptor-related protein 1 in normal tension glaucoma.
Hayashi H, Eguchi Y, Fukuchi-Nakaishi Y, Takeya M, Nakagata N, Tanaka K, Vance JE, Tanihara H
The Journal of biological chemistry 2012 Jul 20;287(30):25395-406
The Journal of biological chemistry 2012 Jul 20;287(30):25395-406
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human LRP-1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human LRP-1 Intracellular Domain Monoclonal Antibody (Catalog # MAB6360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LRP-1 in HepG2 Human Cell Line. LRP-1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human LRP-1 Intracellular Domain Monoclonal Antibody (Catalog # MAB6360) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LRP-1 in Human Liver. LRP-1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human LRP-1 Intracellular Domain Monoclonal Antibody (Catalog # MAB6360) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
