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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX11419 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX11419, RRID:AB_368710
- Product name
- GADD153 antibody [9C8]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Mouse
Submitted references Tanshinone IIA inhibits gastric carcinoma AGS cells through increasing p-p38, p-JNK and p53 but reducing p-ERK, CDC2 and cyclin B1 expression.
Su CC
Anticancer research 2014 Dec;34(12):7097-110
Anticancer research 2014 Dec;34(12):7097-110
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of endogenous GADD153 from primary human fibroblasts using GADD153 Monoclonal Antibody (GTX11419). Lane 1: Untreated cells Lane 2: Cells treated with tunicamycin for 10 hours.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of HeLa cell lysates using GADD153 Monoclonal Antibody (GTX11419). HeLa cells were left untreated (-) or treated with tunicamycin (5?g/ml) for 11 hours (+). Membranes containing whole cell extracts were blocked with 5% milk in TBST (blocking buffer) and probed overnight at 4?C with GTX11419 (diluted 1:2000 in blocking buffer), followed by a goat anti-mouse IgG-HRP secondary antibody (diluted in blocking buffer) and a chemiluminescent substrate. Membranes were also probed for actin as a loading control.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence staining of GADD153 in A549 cells treated with media only (non-treated) or with 1.5 ?M tunicamycin for 6 hours. Cells were stained according to the kit protocol.
