Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF3795 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Methionine Aminopeptidase 2/METAP2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Methionine Aminopeptidase 2/METAP2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human METAP1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P50579
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Pig epiblast stem cells depend on activin/nodal signaling for pluripotency and self-renewal.
Alberio R, Croxall N, Allegrucci C
Stem cells and development 2010 Oct;19(10):1627-36
Stem cells and development 2010 Oct;19(10):1627-36
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Methionine Aminopeptidase 2 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, LS180 human colorectal adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Methionine Aminopeptidase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3795) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Methionine Aminopeptidase 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.