MA5-34830

antibody from Invitrogen Antibodies

Targeting: PSENEN PEN2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34830

Provider

Invitrogen Antibodies

Product name

PEN2 Recombinant Rabbit Monoclonal Antibody (JE41-28)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Mouse spleen tissue, rat cerebellum tissue, human tonsil tissue, human lung cancer tissue, human kidney tissue, mouse testis tissue, A549, SH-SY-5Y.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JE41-28

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PEN2 in mouse spleen tissue lysate. Samples were incubated with PEN2 monoclonal antibody (Product # MA5-34830), at a dilution of 1:2000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PEN2 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PEN2 monoclonal antibody (Product # MA5-34830), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PEN2 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PEN2 monoclonal antibody (Product # MA5-34830), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PEN2 in paraffin-embedded rat cerebellum tissue. Samples were incubated with PEN2 monoclonal antibody (Product # MA5-34830), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PEN2 in paraffin-embedded mouse testis tissue. Samples were incubated with PEN2 monoclonal antibody (Product # MA5-34830), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PEN2 in paraffin-embedded human tonsil tissue. Samples were incubated with PEN2 monoclonal antibody (Product # MA5-34830), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PEN2 in paraffin-embedded human lung cancer tissue. Samples were incubated with PEN2 monoclonal antibody (Product # MA5-34830), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PEN2 in paraffin-embedded human kidney tissue. Samples were incubated with PEN2 monoclonal antibody (Product # MA5-34830), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PEN2 in A549 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with PEN2 monoclonal antibody (Product # MA5-34830) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.