PAB20517

antibody from Abnova Corporation

Targeting: HLA-DQA2 HLA-DXA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20517

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20517, RRID:AB_10965924

Product name

HLA-DQA2 polyclonal antibody

Antibody type

Polyclonal

Antigen

Recombinant protein corresponding to amino acids of human HLA-DQA2.

Reactivity

Human

Host

Rabbit

Antigen sequence

DHVASYGVNFYQSHGPSGQYTHEFDGDEEFYVDLE
TKETVWQLPMFSKFISFDPQSALRNMAVGKHTLEF
MMRQSNSTAATNEVPEVTVFSKFPVTLG


Isotype

IgG

Vial size

100 μl

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with HLA-DQA2 polyclonal antibody (Cat # PAB20517) at 1:250-1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil with HLA-DQA2 polyclonal antibody (Cat # PAB20517) shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)