Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF8279 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal alpha-Actinin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse and rat alpha-Actinin 1 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Disruption of cardiac thin filament assembly arising from a mutation in LMOD2: A novel mechanism of neonatal dilated cardiomyopathy.
Ahrens-Nicklas RC, Pappas CT, Farman GP, Mayfield RM, Larrinaga TM, Medne L, Ritter A, Krantz ID, Murali C, Lin KY, Berger JH, Yum SW, Carreon CK, Gregorio CC
Science advances 2019 Sep;5(9):eaax2066
Science advances 2019 Sep;5(9):eaax2066
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat alpha-Actinin 1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat alpha-Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for alpha-Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.