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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- TA344352 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-ACTN1 Antibody - N-terminal region
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-ACTN1 Antibody - N-terminal region
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ACTN1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB Suggested Anti-ACTN1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample Type: 1.-4. rACTN1-GFP + HEK293 (50ug); Primary Dilution: 1:1000-1:2000; Secondary Antibody: HRP conjugated goat anti-rabbit; Secondary Dilution: 1:20,000; Image Submitted By: Magdalena Kalinowska; Albert Einstein College of Medicine??????
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ACTN1 antibody - N-terminal region validated by WB using 1.-2.Rat cortical neurons ctr siRNA (30ug); 2. Rat cortical neurons ACTN1 siRNA (30ug) at 1:1000-1:2000.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Lanes: Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate; Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate; Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate; Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate; Primary Antibody Dilution: 1:1000; Secondary Antibod
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample Type : ACTNX-GFP transfected COS-7 cellsPrimary Antibody Dilution : 1:1000Secondary Antibody : Anti-rabbit-Alexa-Fluor 568Secondary Antibody Dilution : 1:100Color/Signal Descriptions : Green: GFP Red: ACTN1Gene Name : ACTN1Submitted by : Johannes W
- Validation comment
- IHC
