Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- GTX84959 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84959, RRID:AB_10727698
- Product name
- alpha Actinin antibody [7A4]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
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Supportive validation
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- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 μg per lane) were separated by SDS-PAGE and immunoblotted with anti-ACTN1.
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- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 μg per lane) were separated by SDS-PAGE and immunoblotted with anti-ACTN1.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-ACTN1 mouse monoclonal antibody (GTX84959).
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- GeneTex (provider)
- Main image
- Experimental details
- Anti-ACTN1 mouse monoclonal antibody (GTX84959) immunofluorescent staining of Hela cells transiently transfected with ACTN1
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-ACTN1 mouse monoclonal antibody (GTX84959).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-ACTN1 mouse monoclonal antibody. (GTX84959)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ACTN1 mouse monoclonal antibody. (GTX84959)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ACTN1 mouse monoclonal antibody. (GTX84959)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-ACTN1 mouse monoclonal antibody. (GTX84959)
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- GeneTex (provider)
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- Experimental details
- Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-ACTN1 mouse monoclonal antibody. (GTX84959)