LS-B1445

antibody from LSBio

Targeting: INS IDDM1, IDDM2

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B1445

Provider

LSBio

Product name

IHC-plus™ Insulin Antibody (clone 2D11.H5) LS-B1445

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2D11.H5

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Insulin Antibody - ELISA. ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1ug of conjugate. The starting concentration of antibody in the dilution series was 10 ug/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approximately 5 ng/ml antibody or a 1:200000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L(LS-C60680) and TMB substrate were used for detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-Insulin Antibody - ELISA. ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1ug of conjugate. The starting concentration of antibody in the dilution series was 10 ug/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approximately 5 ng/ml antibody or a 1:200000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L(LS-C60680) and TMB substrate were used for detection.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.