Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- R1530P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1530P, RRID:AB_1004210
- Product name
- anti Insulin
- Antibody type
- Monoclonal
- Antigen
- Purified Human Insulin coupled to Bovine Serum Albumin (BSA).
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2D11.H5
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Dot blotting: Mab anti-Insulin antibody (clone 2D11.H5) is shown to detect human insulin by dot blot. Each dot blot represents 1 µl of non-denatured human insulin at various dilutions starting at 1.0 µg/ml spotted on to nitrocellulose. A 1:400 dilution of Mab anti-Insulin is used for 2 hour followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] reacted for 45 min at room temperature and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- ELISA: Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1µg of conjugate. The starting concentration of antibody in the dilution series was 10 µg/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approx-imately 5ng/ml antibody or a 1:200,000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L and TMB substrate were used for detection.