R1530P

antibody from Acris Antibodies GmbH

Targeting: INS IDDM1, IDDM2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

R1530P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1530P, RRID:AB_1004210

Product name

anti Insulin

Antibody type

Monoclonal

Antigen

Purified Human Insulin coupled to Bovine Serum Albumin (BSA).

Reactivity

Human, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

2D11.H5

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Dot blotting: Mab anti-Insulin antibody (clone 2D11.H5) is shown to detect human insulin by dot blot. Each dot blot represents 1 µl of non-denatured human insulin at various dilutions starting at 1.0 µg/ml spotted on to nitrocellulose. A 1:400 dilution of Mab anti-Insulin is used for 2 hour followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] reacted for 45 min at room temperature and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

ELISA: Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1µg of conjugate. The starting concentration of antibody in the dilution series was 10 µg/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approx-imately 5ng/ml antibody or a 1:200,000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L and TMB substrate were used for detection.