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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- MAB13361 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Proinsulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Proinsulin. Does not detect mature insulin.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01308- Isotype
- IgG
- Antibody clone number
- 253627
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions.
A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice.
Sox17 regulates insulin secretion in the normal and pathologic mouse β cell.
PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance.
Promotion of beta-cell differentiation in pancreatic precursor cells by adult islet cells.
Sambataro M, Sambado L, Trevisiol E, Cacciatore M, Furlan A, Stefani PM, Seganfreddo E, Durante E, Conte S, Della Bella S, Paccagnella A, Dei Tos AP
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jul;32(7):3742-3751
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jul;32(7):3742-3751
A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice.
Tripathi D, Venkatasubramanian S, Cheekatla SS, Paidipally P, Welch E, Tvinnereim AR, Vankayalapati R
Nature communications 2016 Dec 16;7:13896
Nature communications 2016 Dec 16;7:13896
Sox17 regulates insulin secretion in the normal and pathologic mouse β cell.
Jonatan D, Spence JR, Method AM, Kofron M, Sinagoga K, Haataja L, Arvan P, Deutsch GH, Wells JM
PloS one 2014;9(8):e104675
PloS one 2014;9(8):e104675
PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance.
Cao M, Mao Z, Kam C, Xiao N, Cao X, Shen C, Cheng KK, Xu A, Lee KM, Jiang L, Xia J
PLoS biology 2013;11(4):e1001541
PLoS biology 2013;11(4):e1001541
Promotion of beta-cell differentiation in pancreatic precursor cells by adult islet cells.
Chen W, Begum S, Opare-Addo L, Garyu J, Gibson TF, Bothwell AL, Papaioannou VE, Herold KC
Endocrinology 2009 Feb;150(2):570-9
Endocrinology 2009 Feb;150(2):570-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Proinsulin in beta TC-6 Mouse Cell Line. Proinsulin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Mouse Anti-Human/Mouse Proinsulin Monoclonal Antibody (Catalog # MAB13361) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
