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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- MAB1417 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Bovine Insulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects bovine, human, and mouse insulin.
- Reactivity
- Human, Mouse, Bovine
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 182410
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Single-Cell Heterogeneity Analysis and CRISPR Screen Identify Key β-Cell-Specific Disease Genes.
Macrophage contact dependent and independent TLR4 mechanisms induce β-cell dysfunction and apoptosis in a mouse model of type 2 diabetes.
Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarization.
Targeting VEGF-B as a novel treatment for insulin resistance and type 2 diabetes.
Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus.
Conversion of immortal liver progenitor cells into pancreatic endocrine progenitor cells by persistent expression of Pdx-1.
Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress.
Fang Z, Weng C, Li H, Tao R, Mai W, Liu X, Lu L, Lai S, Duan Q, Alvarez C, Arvan P, Wynshaw-Boris A, Li Y, Pei Y, Jin F, Li Y
Cell reports 2019 Mar 12;26(11):3132-3144.e7
Cell reports 2019 Mar 12;26(11):3132-3144.e7
Macrophage contact dependent and independent TLR4 mechanisms induce β-cell dysfunction and apoptosis in a mouse model of type 2 diabetes.
Cucak H, Mayer C, Tonnesen M, Thomsen LH, Grunnet LG, Rosendahl A
PloS one 2014;9(3):e90685
PloS one 2014;9(3):e90685
Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarization.
Cucak H, Grunnet LG, Rosendahl A
Journal of leukocyte biology 2014 Jan;95(1):149-60
Journal of leukocyte biology 2014 Jan;95(1):149-60
Targeting VEGF-B as a novel treatment for insulin resistance and type 2 diabetes.
Hagberg CE, Mehlem A, Falkevall A, Muhl L, Fam BC, Ortsäter H, Scotney P, Nyqvist D, Samén E, Lu L, Stone-Elander S, Proietto J, Andrikopoulos S, Sjöholm A, Nash A, Eriksson U
Nature 2012 Oct 18;490(7420):426-30
Nature 2012 Oct 18;490(7420):426-30
Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus.
Kalis M, Bolmeson C, Esguerra JL, Gupta S, Edlund A, Tormo-Badia N, Speidel D, Holmberg D, Mayans S, Khoo NK, Wendt A, Eliasson L, Cilio CM
PloS one 2011;6(12):e29166
PloS one 2011;6(12):e29166
Conversion of immortal liver progenitor cells into pancreatic endocrine progenitor cells by persistent expression of Pdx-1.
Jin CX, Li WL, Xu F, Geng ZH, He ZY, Su J, Tao XR, Ding XY, Wang X, Hu YP
Journal of cellular biochemistry 2008 May 1;104(1):224-36
Journal of cellular biochemistry 2008 May 1;104(1):224-36
Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress.
Dowling P, O'Driscoll L, O'Sullivan F, Dowd A, Henry M, Jeppesen PB, Meleady P, Clynes M
Proteomics 2006 Dec;6(24):6578-87
Proteomics 2006 Dec;6(24):6578-87
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Insulin in beta TC-6 Mouse Cell Line. Insulin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
