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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- LS-C140548 - Provider product page
- Provider
- LSBio
- Product name
- Anti-STAT3 Antibody (C-Terminus) LS-C140548
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µg
- Storage
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Western Blotting Extracts of NIH3T3 cells stimulated with 50 ng/mL PDGF for 5 minutes were resolved by SDS-PAGE on a 10% Tris-Glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, and then incubated with 1.0 ug/mL of the STAT3 antibody for two hours at room temperature in a 1% milk-TBST buffer. After washing, the membrane was incubated with goat F(ab')2 anti-rabbit IgG HRP-conjugate and signals were detected using the Pierce SuperSignal method.
