Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX24740 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX24740, RRID:AB_367339
- Product name
- STAT3 (phospho Tyr705) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- STAT3 [pY705] (human) phosphospecific antibody. 3T3-L1 cells were stimulated to show specific phosphorylation at tyrosine 705 with IFN, LIF, or PDGF stimulation (upper panel). Pan STAT3 (Cat. no.44-364) is used to show consistent presence of STAT3
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Extracts of 3T3L1 cells unstimulated (1) or stimulated with 20 ng/mL LIF for 10 minutes (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4¢XC, then incubated with the STAT3 [pY705] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the phosphopeptide immunogen (2), a generic phosphotyrosine-containing peptide (3), or the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG HRP-conjugate and signals were detected. The data show that only the phosphopeptide corresponding to STAT3 [pY705] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of STAT3 [pY705] phosphorylation by the addition of LIF in this cell system.
