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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- AMAb90776 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#AMAb90776, RRID:AB_2665662
- Product name
- Anti-STAT3
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
GVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAE
IIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYC
RPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTI
DLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTF
DMELTSECA- Epitope
- Binds to an epitope located within the peptide sequence TKFICVTPTTCSNTI as determined by overlapping synthetic peptides.
- Isotype
- IgG
- Antibody clone number
- CL0490
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-STAT3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Lane 1: Marker [kDa]Lane 2: Human cell line RT-4
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human prostate cancer shows strong nuclear and moderate cytoplasmic immunoreactivity in tumor cells.
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human stomach cancer shows strong nuclear and moderate cytoplasmic positivity in tumor cells.
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human kidney shows strong nuclear combined with moderate cytoplasmic immunoreactivity.
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human rectum shows positivity in glandular cells.
