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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PAB12784 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12784, RRID:AB_10676201
- Product name
- STAT3 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues surrounding S727 (PMSPR) of human, mouse, rat, bovine, chicken and dog STAT3.
- Host
- Rabbit
- Vial size
- 100 µg
- Storage
- Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references A new expression cloning strategy for isolation of substrate-specific kinases by using phosphorylation site-specific antibody.
Matsuo R, Ochiai W, Nakashima K, Taga T
Journal of immunological methods 2001 Jan 1;247(1-2):141-51
Journal of immunological methods 2001 Jan 1;247(1-2):141-51
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The whole cell lysate derived from A-431 was immunoblotted by STAT3 polyclonal antibody (Cat # PAB12784) at 1 : 500.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot Blot : 1 ug peptide was blot onto NC membrane.A : STAT3 (pS727) (Phosphospecific) B : STAT3 (Paired S727) (Nonphosphospeicfic) were blotted at a 1 : 2000 dilution by :1 : STAT3 polyclonal antibody (Cat # PAB12784),2 : Phospho-STAT3 S727 polyclonal antibody (Cat # PAB12753).
- Validation comment
- Dot Blot (Peptide)
