701062

antibody from Invitrogen Antibodies

Targeting: STAT3 APRF

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

701062

Provider

Invitrogen Antibodies

Product name

Phospho-STAT3 (Tyr705) Recombinant Rabbit Monoclonal Antibody (3H16L22)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Antibody clone number

3H16L22

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-STAT3 pTyr705 in whole cell extracts of HeLa treated with IFN1a (150 ng/mL, 15 min, lane 1 and 3) or with Insulin (100 ng/mL, 15 min, lane 2 and 4) using a Phospho-STAT3 pTyr705 recombinant rabbit monoclonal antibody (Product # 701062) at a dilution of 2.5 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lanes 3 and 4). Results show a band at ~90 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Indirect ELISA analysis of endogenous Phospho-STAT3 pTyr705 in HeLa cells (treated with Insulin) coated onto the plate using a Phospho-STAT3 pTyr705 recombinant rabbit monoclonal antibody (Product # 701062) at various dilutions. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody were determined.