Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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Validation data
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- Product number
- 44-384G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-STAT3 (Ser727) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Correction of arginine metabolism with sepiapterin-the precursor of nitric oxide synthase cofactor BH(4)-induces immunostimulatory-shift of breast cancer.
Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch.
The nuclear receptor RORα protects against angiotensin II-induced cardiac hypertrophy and heart failure.
ATM‑JAK‑PD‑L1 signaling pathway inhibition decreases EMT and metastasis of androgen‑independent prostate cancer.
Guanylate binding protein 1 is a novel effector of EGFR-driven invasion in glioblastoma.
The Cdk5/p35 kinases modulate leptin-induced STAT3 signaling.
Early-onset and robust amyloid pathology in a new homozygous mouse model of Alzheimer's disease.
Melanocortin potentiates leptin-induced STAT3 signaling via MAPK pathway.
Overexpression of Wnt-1 in thyrocytes enhances cellular growth but suppresses transcription of the thyroperoxidase gene via different signaling mechanisms.
The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and janus kinase (JAK)/STAT signaling pathways.
Protein tyrosine phosphatase 1B negatively regulates leptin signaling in a hypothalamic cell line.
Protein tyrosine phosphatase 1B negatively regulates leptin signaling in a hypothalamic cell line.
The ribosomal S6 kinases, cAMP-responsive element-binding, and STAT3 proteins are regulated by different leukemia inhibitory factor signaling pathways in mouse embryonic stem cells.
Activation of signal transducers and activators of transcription 1 and 3 by leukemia inhibitory factor, oncostatin-M, and interferon-gamma in adipocytes.
Activation of signal transducers and activators of transcription 1 and 3 by leukemia inhibitory factor, oncostatin-M, and interferon-gamma in adipocytes.
Zheng X, Fernando V, Sharma V, Walia Y, Letson J, Furuta S
Biochemical pharmacology 2020 Jun;176:113887
Biochemical pharmacology 2020 Jun;176:113887
Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch.
Mishra SK, Wheeler JJ, Pitake S, Ding H, Jiang C, Fukuyama T, Paps JS, Ralph P, Coyne J, Parkington M, DeBrecht J, Ehrhardt-Humbert LC, Cruse GP, Bäumer W, Ji RR, Ko MC, Olivry T
Cell reports 2020 Apr 7;31(1):107472
Cell reports 2020 Apr 7;31(1):107472
The nuclear receptor RORα protects against angiotensin II-induced cardiac hypertrophy and heart failure.
Beak JY, Kang HS, Huang W, Myers PH, Bowles DE, Jetten AM, Jensen BC
American journal of physiology. Heart and circulatory physiology 2019 Jan 1;316(1):H186-H200
American journal of physiology. Heart and circulatory physiology 2019 Jan 1;316(1):H186-H200
ATM‑JAK‑PD‑L1 signaling pathway inhibition decreases EMT and metastasis of androgen‑independent prostate cancer.
Zhang L, Xu LJ, Zhu J, Li J, Xue BX, Gao J, Sun CY, Zang YC, Zhou YB, Yang DR, Shan YX
Molecular medicine reports 2018 May;17(5):7045-7054
Molecular medicine reports 2018 May;17(5):7045-7054
Guanylate binding protein 1 is a novel effector of EGFR-driven invasion in glioblastoma.
Li M, Mukasa A, Inda MM, Zhang J, Chin L, Cavenee W, Furnari F
The Journal of experimental medicine 2011 Dec 19;208(13):2657-73
The Journal of experimental medicine 2011 Dec 19;208(13):2657-73
The Cdk5/p35 kinases modulate leptin-induced STAT3 signaling.
He Y, Kastin AJ, Hsuchou H, Pan W
Journal of molecular neuroscience : MN 2009 Sep;39(1-2):49-58
Journal of molecular neuroscience : MN 2009 Sep;39(1-2):49-58
Early-onset and robust amyloid pathology in a new homozygous mouse model of Alzheimer's disease.
Willuweit A, Velden J, Godemann R, Manook A, Jetzek F, Tintrup H, Kauselmann G, Zevnik B, Henriksen G, Drzezga A, Pohlner J, Schoor M, Kemp JA, von der Kammer H
PloS one 2009 Nov 20;4(11):e7931
PloS one 2009 Nov 20;4(11):e7931
Melanocortin potentiates leptin-induced STAT3 signaling via MAPK pathway.
Zhang Y, Wu X, He Y, Kastin AJ, Hsuchou H, Rosenblum CI, Pan W
Journal of neurochemistry 2009 Jul;110(1):390-9
Journal of neurochemistry 2009 Jul;110(1):390-9
Overexpression of Wnt-1 in thyrocytes enhances cellular growth but suppresses transcription of the thyroperoxidase gene via different signaling mechanisms.
Kim WB, Lewis CJ, McCall KD, Malgor R, Kohn AD, Moon RT, Kohn LD
The Journal of endocrinology 2007 Apr;193(1):93-106
The Journal of endocrinology 2007 Apr;193(1):93-106
The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and janus kinase (JAK)/STAT signaling pathways.
Burysek L, Syrovets T, Simmet T
The Journal of biological chemistry 2002 Sep 6;277(36):33509-17
The Journal of biological chemistry 2002 Sep 6;277(36):33509-17
Protein tyrosine phosphatase 1B negatively regulates leptin signaling in a hypothalamic cell line.
Kaszubska W, Falls HD, Schaefer VG, Haasch D, Frost L, Hessler P, Kroeger PE, White DW, Jirousek MR, Trevillyan JM
Molecular and cellular endocrinology 2002 Sep 30;195(1-2):109-18
Molecular and cellular endocrinology 2002 Sep 30;195(1-2):109-18
Protein tyrosine phosphatase 1B negatively regulates leptin signaling in a hypothalamic cell line.
Kaszubska W, Falls HD, Schaefer VG, Haasch D, Frost L, Hessler P, Kroeger PE, White DW, Jirousek MR, Trevillyan JM
Molecular and cellular endocrinology 2002 Sep 30;195(1-2):109-18
Molecular and cellular endocrinology 2002 Sep 30;195(1-2):109-18
The ribosomal S6 kinases, cAMP-responsive element-binding, and STAT3 proteins are regulated by different leukemia inhibitory factor signaling pathways in mouse embryonic stem cells.
Boeuf H, Merienne K, Jacquot S, Duval D, Zeniou M, Hauss C, Reinhardt B, Huss-Garcia Y, Dierich A, Frank DA, Hanauer A, Kedinger C
The Journal of biological chemistry 2001 Dec 7;276(49):46204-11
The Journal of biological chemistry 2001 Dec 7;276(49):46204-11
Activation of signal transducers and activators of transcription 1 and 3 by leukemia inhibitory factor, oncostatin-M, and interferon-gamma in adipocytes.
Stephens JM, Lumpkin SJ, Fishman JB
The Journal of biological chemistry 1998 Nov 20;273(47):31408-16
The Journal of biological chemistry 1998 Nov 20;273(47):31408-16
Activation of signal transducers and activators of transcription 1 and 3 by leukemia inhibitory factor, oncostatin-M, and interferon-gamma in adipocytes.
Stephens JM, Lumpkin SJ, Fishman JB
The Journal of biological chemistry 1998 Nov 20;273(47):31408-16
The Journal of biological chemistry 1998 Nov 20;273(47):31408-16
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extracts prepared from 3T3L1 cells stimulated with LIF were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with STAT3 [pS727] antibody (Product # 44-384G) for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG HRP-conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to STAT3 [pS727] blocks the antibody signal, demonstrating the specificity of the antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STAT3 [pS727] was performed by loading 20 µg of NIH/3T3 (lane1), NIH/3T3 treated for 10 minutes with 50 ng/mL of PDGF (lane2), NIH/3T3 treated for 10 minutes with 200 ng/mL of EGF (lane3), A549 (lane4) and A549 treated for 10 minutes with 200 ng/mL of EGF (lane5) cell lysate using Novex® NuPAGE® 4-12 % Bis-Tris gel (NP0322BOX), XCell SureLock Electrophoresis System (EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® 2 Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. STAT3 [pS727] was detected at ~83 kDa using STAT3 [pS727] Rabbit Polyclonal Antibody (44384G) at 1:1000 dilution in 5% skim milk at 4ºC overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of STAT3 [pS727] showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a STAT3 [pS727] Rabbit Polyclonal Antibody (44384G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP- qPCR analysis of STAT3 [pSer727] was performed with 10 µL of the STAT3 [pSer727] Rabbit polyclonal antibody (44384G) on sheared chromatin from 2 million HeLa cells treated with 100 ng/mL of IFN Alpha for one hour using the MAGnify Chromatin Immunoprecipitation System (49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA from each ChIP sample was analyzed by StepOnePlus Real-Time PCR System (4376600) with primers for the promoter of active GLS1 gene, used as positive control target, and the cFOS, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL