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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- TA302174 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA302174, RRID:AB_2255569
- Product name
- Rabbit Polyclonal Antibody against STAT3 (Phospho-STAT3-Y727 ) (Phospho-Specific)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Antibody against STAT3 (Phospho-STAT3-Y727 ) (Phospho-Specific)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- STAT3
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.25mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- The phospho-STAT3-Ser727 Pab (Cat. #TA302174) is used in Western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- The phospho-STAT3-Ser727 Pab (Cat. #TA302174) is used in Western blot to detect Ser727-phosphorylated STAT3 in HL-60 tissue lysates collected before (-) or after (+) stimulation obtained by IFN-a.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Phospho-STAT3-S727 Antibody(Cat#TA302174) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of SY5Y cells stained with phospho-STAT3-S727 antibody. SY5Y cells were incubated with TA302174 phospho-STAT3-S727 primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the phospho-STAT3-S727 immunosignal to the nucleus and cytoplasm.
- Validation comment
- IF
