MA5-15712

antibody from Invitrogen Antibodies

Targeting: STAT3 APRF

ELISA

Antibody data

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Validation data

Product number: MA5-15712 - Provider product page
Provider: Invitrogen Antibodies
Product name: STAT3 Monoclonal Antibody (3B5)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15712 targets STAT3 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples. The MA5-15712 immunogen is purified recombinant fragment of human STAT3 expressed in E. Coli. MA5-15712 detects STAT3 which has a predicted molecular weight of approximately 88kDa.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 3B5
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

STAT3 protein structure - MA5-15712 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of STAT3 was achieved by transfecting HeLa with STAT3 specific validated siRNAs (Silencer® select Product # s740). Western blot analysis (Fig. a) was performed using whole cell extracts from the STAT3 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with STAT3 Polyclonal Antibody (Product # MA5-15712, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to STAT3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of STAT3 using STAT3 monoclonal antibody (Product # MA5-15712) in HeLa (1), NIH/3T3 (2), Jurkat (3), PC-12 (4) and COS-7 (5) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of STAT3 (Fig. a) was performed by loading 20 ug of HeLa Control (lane 1), HeLa STAT3 knockout (lane 2) whole cell extracts. STAT3 was detected at 83kDa using STAT3 Monoclonal Antibody (3B5) (Product # MA5-15712, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution) Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal in CRISPR mediated knockout (KO) confirms that antibody is specific to STAT3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of STAT3 using STAT3 monoclonal antibody (Product # MA5-15712) in HeLa (1), NIH/3T3 (2), Jurkat (3), PC-12 (4) and COS-7 (5) cell lysate.