Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AF21671 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal STAT6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse STAT6 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human STAT6 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 30 minutes. Gels were loaded with 20 μg of cytoplasmic (Cyto) and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse STAT6 aa 342-640 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21671) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for STAT6 was detected at approximately 110 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human Phospho-STAT6 (Y641) Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF3717). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.