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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP2-20506 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal STAT6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STAT6 Antibody [NBP2-20506] - Sample (30 ug of whole cell lysate), HepG2 7. 5% SDS PAGE gel, diluted at 1:1000. Note: band was detected at a molecular weight slightly higher than the predicted MW of 94 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STAT6 Antibody [NBP2-20506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT6 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: STAT6 Antibody [NBP2-20506] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of STAT6 protein by 2 ug of STAT6 antibody 10% SDS-PAGE The immunoprecipitated STAT6 protein was detected by STAT6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STAT6 Antibody [NBP2-20506] - C2C12 xenograft, using STAT6 antibody antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STAT6 Antibody [NBP2-20506] - Mouse kidney. STAT6 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
