PAB29361

antibody from Abnova Corporation

Targeting: DNAJA1 dj-2, hdj-2, HSJ2, HSPF4, NEDD7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29361

Provider

Abnova Corporation

Product name

DNAJA1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human DNAJA1.

Antigen sequence

DQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPIS
TLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRR
PYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPE
RKEVEETDEMDQVELVDFDPNQERRRHYNGE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with DNAJA1 polyclonal antibody (Cat # PAB29361) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with DNAJA1 polyclonal antibody (Cat # PAB29361) at 1-4 ug/mL concentration shows positivity in cytoplasm and microtubules.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with DNAJA1 polyclonal antibody (Cat # PAB29361) shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)