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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- ADI-SPA-405-050 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-SPA-405-050, RRID:AB_11179360
- Product name
- HSP40/Hdj2 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full length protein
- Description
- Protein A-affinity purified.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
- Vial size
- 50 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Basal and stress-induced Hsp70 are modulated by ataxin-3.
Methylglyoxal alters the function and stability of critical components of the protein quality control.
Reina CP, Nabet BY, Young PD, Pittman RN
Cell stress & chaperones 2012 Nov;17(6):729-42
Cell stress & chaperones 2012 Nov;17(6):729-42
Methylglyoxal alters the function and stability of critical components of the protein quality control.
Bento CF, Marques F, Fernandes R, Pereira P
PloS one 2010 Sep 24;5(9):e13007
PloS one 2010 Sep 24;5(9):e13007
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Supportive validation
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- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western Blot Analysis of HSP40/Hdj2, pAb: Lane 1: MWM, Lane 2: Hsp40/Hdj2 Recombinant Human Protein (Prod. No. ADI-SPP-405), Lane 3: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100), Lane 4: HeLa Cell Lysate, Heat Shocked (Prod. No. ADI-LYC-HL101), Lane 5: PC-12 Cell Lysate (Prod. No. ADI-LYC-PC100), Lane 6: PC-12 Cell Lysate, Heat Shocked (Prod. No. ADI-LYC-PC101), Lane 7: 3T3 Cell Lysate (Prod. No. ADI-LYC-3T100), Lane 8: 3T3 Cell Lysate, Heat Shocked (Prod. No. ADI-LYC-3T101), Lane 9: CHO-K1 Cell Lysate, Lane 10: CHO-K1 Cell Lysate, Heat Shocked.
