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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA1-19390 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Zap-70 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody recognizes the C-terminal part of human ZAP-70 protein tyrosine kinase. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human ZAP70 using rabbit polyclonal antibody PAb (430) (Product # PA1-19390) on lysates of MOLT-4 cell line and HEK293T/17 cell line (ZAP70 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 5µg/mL of rabbit anti-ZAP70 polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for ZAP70 protein at approximately 70kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human ZAP70 using rabbit polyclonal antibody PAb (430) (Product # PA1-19390) on lysates of MOLT-4 cell line and HEK293T/17 cell line (ZAP70 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 5µg/mL of rabbit anti-ZAP70 polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for ZAP70 protein at approximately 70kDa.
