AP0080

antibody from Bioworld Technology, Inc
Targeting: ZAP70 SRK, STD, ZAP-70
Provider product page for AP0080

Antibody data

Product number
AP0080 - Provider product page
Provider
Bioworld Technology, Inc
Product name
ZAP70 (phospho Tyr292) polyclonal antibody
Antibody type
Polyclonal
Antigen
Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of Tyrosine 292.
Description
The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes . The TCR subunits include the Ti a and b chains, the CD3 g , d and e chains and a z -containing homodimer or heterodimer . The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR z chain through two src-homology (SH2) domains . This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr292 and Tyr319 . ZAP-70 is autophosphorylated on Tyr292, which is thought to negatively regulate ZAP-70 function in lymphocytes . Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr319, which mediates the SH2-dependent interaction between Lck and ZAP-70 .
Reactivity
Human, Rat
Host
Rabbit
Isotype
IgG
Vial size
100ul
Concentration
1 mg/ml
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
ZAP70 protein structure - AP0080 shown in red.