ARG65562

antibody from Arigo

Targeting: ZAP70 SRK, STD, ZAP-70

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

ARG65562

Provider

Arigo

Product name

anti-ZAP-70 antibody [ZAP-03]

Antibody type

Monoclonal

Antigen

Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag

Description

Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

ZAP-03

Vial size

100 µg

Concentration

1 mg/ml

Storage

Aliquot and store at 2-8°C. Do not freeze. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western Blot: HPB-ALL peripheral blood T cell leukemia cell lysate stained with antibody clone ZAP-03 1-4. stained with anti-ZAP-70 ( 4 µg/ml , 2 µg/ml, 1 µg/ml, 0.5 µg/ml respectively), 5. stained with anti-ZAP-70 comparative antibody, 6. stained with Isotype mouse IgG1 control

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western Blot: 1,3. HPB-ALL peripheral blood T cell leukemia cell lysate, 2,4 RAMOS cell lysate stained with antibody clone ZAP-03 Lane 1, 2: stained with anti-ZAP-70 (ZAP-03; 0.5 µg/ml), Lane 3, 4: stained with Isotype mouse IgG1 control